In vitro anthelmintic effect of Anogeissus leiocarpus (DC.) guill. & perr. leaf extracts and fractions on developmental stages of Haemonchus contortus

摘要

The anthelmintic effect of acetone leaf extract and fractions of Anogeissus leiocarpus was investigated to determine therelative efficacy of the components as anthelmintic against Haemonchus contortus (Rudolphi). The fractions were obtained bysolvent-solvent group separation of the leaf extract. The fractions were evaluated for ovicidal and larvicidal activity by egg hatchinhibition assay and larval development viability assay. Best-fit LC50 values for egg hatch test were 0.360, 0.316, 0.093, 0.219and 0.196 mg/ml for the crude acetone extract, hexane, chloroform, butanol, and 35% water in methanol fractions, respectively.While the best-fit LC50 values for larval development and viability test were 0.509, 0.162, 0.186, 0.288 and 0.130 mg/ml for thecrude acetone extract, hexane, chloroform, butanol, and 35% water in methanol fractions, respectively. The 35% water inmethanol fractions was the more active on larvae, although differences in activity between fractions were not significant(p>0.05). A. leiocarpus leaf extracts could find application in anthelmintic therapy in veterinary practice.